Isolation and identification of Murine Mesenchymal Stem Cells from Bone Marrow.

Background: Mesenchymal stem cells (MSCs) are beneficial for cell and gene therapy approaches. These highly potent in producing various cells, including adipocytes, osteoblasts, hematopoietic etc., particularly bone marrow-derived. High proliferation regenerative capacity important phenotypes of MSCs which contribute to the recovery process at damaged sites. Various techniques have been used, inconvenient effects on stromal properties. The present study evaluates markers isolated from Murine mouse marrow. Methodology: murine mesenchymal (mMSCs) were marrow Balb/c mice using a simple vitro culture protocol. Trypan blue exclusion assay was performed viable count, cellular morphology checked an inverted microscope. immunofluorescence staining done analyze immunophenotypic features mMSCs by treating with CD44, CD90, CD45 fluorescent antibodies. Results: spindle-like appearance attained within three weeks. Immunofluorescence displayed statistically significant (p<0.001) number CD44 CD90 positive cells. Alternatively, insignificant count labeled found. Conclusion: A uniform mMSC population achieved high propagation capability lower passages through regular replacement medium reduced trypsinization time. In addition, strongly expressed markers, CD90.